p53 Antibody / TP53

• Catalog Number: orb750267
• Category: Antibodies
• Description: p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant human wild type p53 protein was used as the immunogen for this anti-p53 antibody. Its epitope maps within the N-terminus (approx. amino acids 20-25).
• UniProt ID: P04637
• Tested applications: IHC-P, WB
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Application notes: Optimal dilution of the antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: SPM589
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This anti-p53 antibody is available for research use only.
• Note: For research use only

Western blot testing of human HeLa cell lysate with anti-p53 antibody (clone SPM589). Expetected molecular weight ~53 kDa.

IHC: Formalin-fixed, paraffin-embedded human bladder carcinoma stained with anti-p53 antibody (clone SPM589).