TP53 Antibody

SKU: orb344399

Description

p53 antibody

Images & Validation

• Tested Applications: ChIP, ELISA, IF, IHC, IP, Multiplex Assay, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, ChIP: 1 µg/µL at 4° o/n, IHC: 1:50, IF: 1:100-1:500, IP: 1:100, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  Anti-p53 has been tested by western blot and immunofluorescence and is suitable for ChIP, flow cytometry, immunohistochemistry, Immunofluorescence, immunoblotting and immunoprecipitation. p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with an N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.

  Read more...

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: BP53-12
• Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
• Target: TP53
• Purity: This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines. The antibody recognizes a 53 kDa band corresponding to p53. Cross reactivity with p53 from other sources has not been determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

mouse anti-p53 antibody, mouse anti-Tumor Suppressor p53 antibody, Phosphoprotein p53 antibody, TP53 antibody, Transformation related protein 53 antibody, TRP53 antibody, cellular Tumor antigen p53 antibody

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

RefSeq:23491729