P53 Antibody

SKU: orb1925686

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: IHC - 1:400, WB - 1:2000
• Reactivity: Human, Monkey

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone No.: B3933EV306X30X3
• Immunogen: This P53 antibody is generated from a mouse immunized with a recombinant protein conjugated synthetic peptide between 1-393 amino acids from human P53.
• Target: TP53
• Molecular Weight: 43653 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Immunohistochemical analysis of paraffin-embedded Human Colon cancer section using Pink1. Diluted at 1:400 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

All lanes: Anti-P53 Antibody at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lane 3: CCRF-CEM whole cell lysate. Lane 4: COS-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637