p53 Antibody

SKU: orb1330851

Description

Mouse monoclonal antibody to p53 (TP53)

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB 1:2000, IHC 1:150
• Reactivity: Human
• Application Notes:
  For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: DO-1
• Immunogen: Recombinant human wild type p53 protein expressed in E. coli.
• Target: p53
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (Right lane) cDNA for 48 hrs and lysed using p53 (TP53) antibody

Quick Database Links

Gene Symbol

p53

UniProt

P04637

RefSeq (mRNA):NM_000546