TP53 Antibody

SKU: orb1261874

Description

TP53 Antibody

Research Area

Cancer Biology, Cell Biology, Epigenetics & Chromatin, Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: ChIP, IF, IHC, WB
• Reactivity: Human, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200ChIP: 1:50 - 1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human p53 (NP_000537.3).
• Target: TP53
• Molecular Weight: Observed: 53kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

TP53, P53, BCC7, LFS1, TRP53

Immunofluorescence analysis of A549 cells using p53 antibody.

Western blot analysis of extracts of various cell lines, using p53 antibody at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human colon carcinoma using p53 antibody at dilution of 1:200 (40x lens).

Chromatin immunoprecipitation analysis of extracts of 293T cells, using p53 antibody and rabbit IgG.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637