p53 antibody

SKU: orb2276234

Description

p53 antibody validated for immunohistochemistry on 76 different Normal Tissues

Research Area

Cancer Biology

Images & Validation

• Tested Applications: IHC
• Dilution Range: 1:100-1:200
• Reactivity: Human
• Application Notes:
  Positive Control: Tonsil: More than 20 % of germinal centre B-cells must show a weak to moderate nuclear staining. Negative Control: Colon: Luminal epithelial cells must remain p53 negative. Cellular Localization: Intracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

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Key Properties

• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: MSVA-053R
• Immunogen: Recombinant human full-length TP53 protein
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antigen NY-CO-13, BCC7, Cellular Tumor Antigen p53, LFS1, TP53, Transformation Related Protein 53 (TRP53), Tumor Protein p53, Tumor Suppressor p53

Ovary Serous high grade carcinoma with strong nuclear p53 positivity of all tumor cells p53 immunohistochemistry.

Skin Weak nuclear p53 immunostaining of a fraction of basal and suprabasal cells p53 immunohistochemistry scaled.

Urinary bladder Strong nuclear p53 immunostaining of all tumor cells of a muscle invasive urothelial carcinoma p53 immunohistochemistry.

Quick Database Links

UniProt

P04637