TP53 Antibody

SKU: orb1252126

Description

TP53 Antibody

Research Area

Cancer Biology, Cell Biology, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human
• Application Notes:
  Immunofluorescence: 1-2 µg/mLWestern blot: 1-2 µg/mLIHC (FFPE): 1-2 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)Titering of the p53 antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: CTA53-1
• Immunogen: Human recombinant protein was used as the immunogen for this p53 antibody.
• Target: TP53
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Concentration: 0.2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Western blot testing of human samples with p53 antibody (CTA53-1).

IHC staining of human colon carcinoma with p53 antibody (CTA53-1).

Quick Database Links

Gene Symbol

TP53

UniProt

P04637