p53 Antibody (C-term)

SKU: orb1930145

This product can be custom made

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cancer Biology

Images & Validation

−

Item 1 of 2

Tested Applications	WB
Dilution Range	WB - 1:2000
Reactivity	Human

Key Properties

−

Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-385 amino acids from the C-terminal region of human p53. Antigen Region: 354-385 aa.
Target	TP53
Molecular Weight	43653 Da
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Form/Appearance	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

All lanes: Anti-p53 Antibody (C-term) at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HT-29 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-p53 Antibody (S376) (p53-S378) at 1:1000-1:2000 dilution. Lane 1: 293T/17 whole cell lysate. Lane 2: A431 whole cell lysate. Lane 3: SK-BR-3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 44 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

TP53

UniProt

P04637

RefSeq (Protein):NP_001119587.1, NP_001119589.1, NP_001263627.1, NP_001119585.1, NP_001119588.1, NP_000537.3, NP_001119584.1, NP_001263628.1, NP_00126, NP_001119586.1, NP_001263625.1, NP_001263626.1, NP_001119590.1, NP_001263624.1

UniProt Details

−

No UniProt data available

NCBI Reference Sequences

−

Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_001119587.1, NP_001119589.1, NP_001263627.1, NP_001119585.1, NP_001119588.1, NP_000537.3, NP_001119584.1, NP_001263628.1, NP_00126, NP_001119586.1, NP_001263625.1, NP_001263626.1, NP_001119590.1, NP_001263624.1

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

Find More Protocols