Sestrin-1 Antibody (C-term)

SKU: orb1929027

Description

Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Cell Biology, Metabolism Research

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB - 1:1000, FC - 1:10-50
• Reactivity: Human, Mouse
• Predicted Reactivity: Other

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: BTD30823
• Immunogen: This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-341 amino acids from the C-terminal region of human Sestrin-1. Antigen Region: 312-341 aa.
• Target: SESN1 (HGNC:21595)
• Molecular Weight: 56557 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Sestrin-1, p53-regulated protein PA26, SESN1, PA26, SEST1

Western blot analysis of Sestrin-1 antibody (C-term) in mouse heart tissue lysates (35 ug/lane). Sestrin-1 (arrow) was detected using the purified Pab.

Sestrin-1 Antibody (C-term) western blot analysis in NCI-H460, HL-60 cell line lysates (35 ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).

Sestrin-1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

SESN1 (HGNC:21595)

UniProt

Q9Y6P5

RefSeq (Protein):NP_001186863.1, NP_001186862.1, NP_055269.1