Sestrin-1 Antibody (C-term)

• Catalog Number: orb1929027
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18601
• Tested applications: FC, WB
• Predicted Reactivity: Other
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 56557 Da
• Target: This Sestrin-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-341 amino acids from the C-terminal region of human Sestrin-1.
• UniProt ID: Q9Y6P5
• NCBI: NP_055269.1, NP_001186863.1, NP_001186862.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Sestrin-1, p53-regulated protein PA26, SESN1, PA26
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Sestrin-1 antibody (C-term) in mouse heart tissue lysates (35 ug/lane). Sestrin-1 (arrow) was detected using the purified Pab.

Sestrin-1 Antibody (C-term) western blot analysis in NCI-H460, HL-60 cell line lysates (35 ug/lane).This demonstrates the Sestrin-1 antibody detected the Sestrin-1 protein (arrow).

Sestrin-1 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.