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p53 (2D10) Mouse mAb

SKU: orb1563238

Description

p53 (2D10) Mouse mAb

Research Area

Cancer Biology

Images & Validation

Tested ApplicationsICC, IF, IHC-Fr, IHC-P, WB
Dilution RangeWB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200
ReactivityHuman

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeIgG1
Clone No.B4F32X3G1X3F5
ImmunogenSynthetic Peptide of p53
TargetTP53
Molecular WeightCalculated MW: 44 kDa; Observed MW: 53 kDa
PurificationAffinity Purified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesLiquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

TP53; P53; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

p53 (2D10) Mouse mAb

Immunofluorescence analysis of p53 in Hela using p53 (2D10) antibody (Left) and DAPI (Right).

p53 (2D10) Mouse mAb

Immunohistochemistry analysis of paraffin-embedded Human colon cancer tissue using p53 (2D10) antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

p53 (2D10) Mouse mAb

Western blot analysis of p53 (2D10) in T47D and COS7 lysates using p53 (2D10) antibody.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC-P
Immunohistochemistry Paraffin
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IHC-Fr
Immunohistochemistry Frozen
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IF
Immunofluorescence
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ICC
Immunocytochemistry
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p53 (2D10) Mouse mAb (orb1563238)

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Available Sizes

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20 μl
$ 130.00
50 μl
$ 220.00
100 μl
$ 370.00
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