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Catalog Number | orb420315 |
---|---|
Category | Antibodies |
Description | p44 Map Kinase antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IP, WB |
Reactivity | Mouse |
Isotype | IgG |
Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the carboxy terminal end of human p44 MAP Kinase (ERK1) protein. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:2,000 to 1:10,000, IP: 5.0 µg/mg sample, WB: 1:1,000-1:,2000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This affinity-purified antibody is directed against the human p44 MAP Kinase (ERK1) protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with p44 MAP Kinase (ERK1) proteins from human, bovine, rabbit, rat and mouse. No reactivity is observed against p42 MAP Kinase (ERK2). Reactivity against homologues from other sources is not known. |
Conjugation | Unconjugated |
UniProt ID | P27361 |
NCBI | 91718899 |
Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | ERK1 antibody, ERT 2 antibody, ERT2 antibody, Extr Read more... |
Note | For research use only |
Application notes | This affinity purified antibody has been tested for use in ELISA, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 44 kDa in size corresponding to p44 MAP Kinase (ERK1) by western blotting in the appropriate cell lysate or extract. |
Expiration Date | 12 months from date of receipt. |
ELISA, IF, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IHC-P, WB | |
Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
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