p27Kip1 Antibody

SKU: orb385809

Description

This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KIP27-1
• Immunogen: Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human prostate cancer and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE human colon carcinoma and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

IHC testing of FFPE rat colon tissue and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

FACS testing of Jurkat cells and Alexa Fluor 488-labeled p27Kip1 antibody.

Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP27-1, green) and Phalloidin (red).

Quick Database Links

UniProt

P46527