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Catalog Number | orb385809 |
---|---|
Category | Antibodies |
Description | This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | KIP27-1 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the p27Kip1 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Flow Cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1) |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This p27Kip1 antibody is available for research use only. |
UniProt ID | P46527 |
Storage | Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human prostate cancer and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human colon carcinoma and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE rat colon tissue and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.
FACS testing of Jurkat cells and Alexa Fluor 488-labeled p27Kip1 antibody.
Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP27-1, green) and Phalloidin (red).
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |