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p27 Antibody / p27Kip1

Catalog Number: orb385809

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb385809
CategoryAntibodies
DescriptionThis mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.
Species/HostMouse
ClonalityMonoclonal
Clone NumberKIP27-1
Tested applicationsFACS, IF, IHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenRecombinant human protein was used as the immunogen for the p27Kip1 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow Cytometry: 1-2ug/million cells in 0.1ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis p27Kip1 antibody is available for research use only.
UniProt IDP46527
StorageStore the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the p27Kip1 antibody to be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
p27 Antibody / p27Kip1

IHC testing of FFPE human prostate cancer and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

p27 Antibody / p27Kip1

IHC testing of FFPE human colon carcinoma and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

p27 Antibody / p27Kip1

IHC testing of FFPE rat colon tissue and p27Kip1 antibody (clone KIP27-1). Staining of formalin-fixed tissues requires boiling tissue sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

p27 Antibody / p27Kip1

FACS testing of Jurkat cells and Alexa Fluor 488-labeled p27Kip1 antibody.

p27 Antibody / p27Kip1

Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP27-1, green) and Phalloidin (red).

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    FACS,  IF,  IHC-P

    Human, Mouse, Rat

    Mouse

    Monoclonal

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