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Catalog Number | orb749446 |
---|---|
Category | Antibodies |
Description | This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | KIP1/769 |
Tested applications | FACS, IF, IHC-P |
Reactivity | Human, Mouse, Rat |
Isotype | Mouse IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the p27Kip1 antibody. |
Antibody Type | Primary Antibody |
Dilution range | Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This p27Kip1 antibody is available for research use only. |
UniProt ID | P46527 |
Storage | Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (clone KIP1/769).
IHC: Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (clone KIP1/769).
IHC: Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 (clone KIP1/769).
Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: Alexa Fluor 488-labeled p27Kip1 antibody (clone KIP1/769).
IHC: Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (clone KIP1/769).
Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP1/769, green) and Phalloidin (red).
SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/769) as confirmation of integrity and purity.
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P | |
Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |