p27Kip1 Antibody

SKU: orb749446

Description

This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Optimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KIP1/769
• Immunogen: Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (clone KIP1/769).

IHC: Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (clone KIP1/769).

IHC: Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 (clone KIP1/769).

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: Alexa Fluor 488-labeled p27Kip1 antibody (clone KIP1/769).

IHC: Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (clone KIP1/769).

Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP1/769, green) and Phalloidin (red).

SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/769) as confirmation of integrity and purity.

Quick Database Links

UniProt

P46527