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p27 Antibody / p27Kip1

Catalog Number: orb749446

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb749446
CategoryAntibodies
DescriptionThis mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This mAb is excellent for staining of formalin-fixed tissues.
Species/HostMouse
ClonalityMonoclonal
Clone NumberKIP1/769
Tested applicationsFACS, IF, IHC-P
ReactivityHuman, Mouse, Rat
IsotypeMouse IgG1, kappa
ImmunogenRecombinant human protein was used as the immunogen for the p27Kip1 antibody.
Antibody TypePrimary Antibody
Dilution rangeFlow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis p27Kip1 antibody is available for research use only.
UniProt IDP46527
StorageStore the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesOptimal dilution of the p27Kip1 antibody to be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
p27 Antibody / p27Kip1

IHC: Formalin-fixed, paraffin-embedded human colon carcinoma stained with p27Kip1 antibody (clone KIP1/769).

p27 Antibody / p27Kip1

IHC: Formalin-fixed, paraffin-embedded human prostate cancer stained with p27Kip1 antibody (clone KIP1/769).

p27 Antibody / p27Kip1

IHC: Formalin-fixed, paraffin-embedded human cervical cancer stained with p27Kip1 (clone KIP1/769).

p27 Antibody / p27Kip1

Flow cytometry testing of Jurkat cells. Black: cells alone; Grey: isotype control; Green: Alexa Fluor 488-labeled p27Kip1 antibody (clone KIP1/769).

p27 Antibody / p27Kip1

IHC: Formalin-fixed, paraffin-embedded rat colon stained with p27Kip1 antibody (clone KIP1/769).

p27 Antibody / p27Kip1

Immunofluorescent staining of PFA-fixed human MCF7 cells with p27Kip1 antibody (clone KIP1/769, green) and Phalloidin (red).

p27 Antibody / p27Kip1

SDS-PAGE analysis of purified, BSA-free p27Kip1 antibody (clone KIP1/769) as confirmation of integrity and purity.

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    FACS,  IF,  IHC-P

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