Ovalbumin antibody (Peroxidase)

• Catalog Number: orb344856
• Category: Antibodies
• Description: Ovalbumin antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Ovalbumin Antibody Peroxidase Conjugat
• Note: For research use only
• Application notes: Anti-Ovalbumin Antibody Peroxidase Conjugated has been tested by ELISA and western blot and is suitable for IHC and IP. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ~1 ug Ovalbumin protein reduced (Lane1), 0.25 ug Ovalbumin protein reduced (Lane2), ~1 ug Ovalbumin protein non-reduced (Lane3), 0.25 ug Ovalbumin protein non-reduced (Lane4) using Ovalbumin antibody (Peroxidase)