Ovalbumin antibody (Peroxidase)

• Catalog Number: orb344855
• Category: Antibodies
• Description: Ovalbumin antibody (Peroxidase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Gallus
• Isotype: IgG
• Immunogen: Anti-Ovalbumin Antibody was produced by repeated immunizations with hen egg white Ovalbumin protein.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IHC: 1:500 - 1:2,500, IP: 1:100, WB: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Ovalbumin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Ovalbumin [Hen Egg White]. Cross reactivity against Ovalbumin from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP
• UniProt ID: P01012
• NCBI: P01012.2
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: rabbit anti-Ovalbumin Antibody Peroxidase Conjugat
• Note: For research use only
• Application notes: Anti-Ovalbumin Antibody Peroxidase Conjugated has been tested by ELISA and western blot and is suitable for IHC and IP. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ~1 ug Ovalbumin protein reduced (Lane1), 0.25 ug Ovalbumin protein reduced (Lane2), ~1 ug Ovalbumin protein non-reduced (Lane3), 0.25 ug Ovalbumin protein non-reduced (Lane4) using Ovalbumin antibody (Peroxidase)