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Osteopontin Rabbit Polyclonal Antibody

Catalog Number: orb11191

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb11191
CategoryAntibodies
DescriptionOsteopontin Rabbit Polyclonal Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Predicted ReactivityRat
ReactivityHuman, Rat
IsotypeIgG
ImmunogenKLH conjugated synthetic peptide derived from human OPN (141-220/314aa)
Antibody TypePrimary Antibody
Concentration1mg/ml
Dilution rangeWB=1:500-2000, IHC-P=1:500-2000, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1ug/Test
Form/AppearanceLiquid
ConjugationUnconjugated
MW34 kDa
TargetSPP1
UniProt IDP10451
RRIDAB_10752366
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Alternative namesBNSP; Bone sialoprotein 1; BSP I; BSPI; Early T ly
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NoteFor research use only
Expiration Date12 months from date of receipt.
Osteopontin Rabbit Polyclonal Antibody

Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (orb11191), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Osteopontin Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Rat lung, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Osteopontin Polyclonal Antibody, Unconjugated (orb11191) at 1:400 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Osteopontin Rabbit Polyclonal Antibody

Sample: A549 Cell Lysate at 40 ug, Primary: Anti-ostepontin (orb11191) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 50 kD.

Osteopontin Rabbit Polyclonal Antibody

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: A549 (Human) Cell Lysate at 30 ug, Lane 3: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-Osteopontin at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 60-65 kD, Observed band size: 60 kD.

  • Osteopontin Rabbit Polyclonal Antibody [orb11192]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Human, Rat

    Human, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • Osteopontin Rabbit Polyclonal Antibody [orb500935]

    FC,  IF,  IHC-Fr,  IHC-P,  WB

    Human

    Human, Rat

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 200 μl, 50 μl
  • OPN-a/b Antibody (N-term) [orb1938103]

    IF,  IHC-P,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl, 50 μl
  • SPP1 Rabbit Polyclonal Antibody [orb576067]

    IHC,  WB

    Bovine, Canine, Goat, Guinea pig, Mouse, Rat, Sheep

    Human

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • OPN rabbit pAb [orb765958]

    ELISA,  IHC-P,  WB

    Human, Mouse, Rat

    Polyclonal

    Unconjugated

    100 μl, 50 μl