Ophiopogonin-D

SKU: orb1300228

Description

Ophiopogonin-D

Research Area

Immunology & Inflammation

Key Properties

• CAS Number: 41753-55-3
• MW: 855.02
• Purity: 97.16%
• Formula: C₄₄H₇₀O₁₆
• SMILES: C[C@H]1[C@H]2[C@H](C[C@H]3[C@@H]4CC=C5C[C@@H](O)C[C@@H](O[C@@H]6O[C@H](C)[C@H](O)[C@H](O[C@@H]7OC[C@@H](O)[C@H](O)[C@H]7O)[C@H]6O[C@@H]6O[C@@H](C)[C@H](O)[C@@H](O)[C@H]6O)[C@]5(C)[C@H]4CC[C@]23C)O[C@]11CC[C@@H](C)CO1
• Target: ROS
• Solubility: DMSO:30 mg/mL (35.09 mM)

Bioactivity

• In Vivo:
  Ophiopogonin D(OPD)′ treatment led to significant tumor growth Inhibition at a dose of 5.0 mg/kg bodyweight, beginning on Day 6 of treatment (p = 0.034). At the end of the study (Day 24) the tumor tissues were excised, photographed, and weighed. Treatment with 5.0 mg/kg bodyweight OPD′ resulted in significant (p = 0.000) tumor growth Inhibition by approximately 79.8% on Day 24 compared to the Vehicle treatment the 5.0 mg/kg dose led to significantly stronger tumor growth Inhibition tha the 2.5 mg/kg dose (p = 0.000). There was not a significant loss of body weight in any of the group.
• In Vitro:
  Ophiopogonin D(OPD) was shown to exert potent anti-tumor activity against PC3 cells. It induced apoptosis via a RIPK1-related pathway, increase the protein expression levels of RIPK1 and Bim, and decrease the levels of cleaved-RIPK1, caspase 8, cleaved-caspase 8, Bid, caspase 10, and cleaved-caspase 10. OPD also increase the mRNA expression of Bim the protein expression of Bim was decreased when cells were pre-treated with necrostatin-.
• Cell Research:
  The CCK-8 assay was used to asses the viability of prostate cancer cells the cell morphology was examined by an ultrastructural analysis via transmission electron microscopy. Cells in apoptosis (early and late stages) were detected using an Annexin V-FITC/propidium iodide kit with a FACSCaliber flow cytometer. JC-1, a cationic lipophilic probe, was employed to measure the mitochondrial membrane potential (MMP) of PC3 cells. Changes in the protein expression of RIPK1, C-RIPK1, caspase 8, cleaved-caspase 8, Bim, Bid, caspase 10, and cleaved-caspase 10 were evaluated by Western blotting the mRNA expression of Bim was examined by quantitative real-time reverse transcription polymerase chain reaction. Z-VAD-FMK (a caspase inhibitor) and necrostatin-1 (a specific inhibitor of RIPK1) were utilized to determine whethe the cell death was mediated by RIPK1 or caspase.
• Animal Research:
  One week after tumor cell inoculation, mice bearing palpable tumors were andomly divided into control and treatment groups (8 mice/group). OPD was dissolved in the Vehicle, PEG400:Saline:Ethanol (400:300:200, v/v/v), and administered (via i.p. injection) at doses of 2.5 or 5.0 mg/kg bodyweight 5 days a week for 24 days the control group received Vehicle only the mice were sacrificed by cervical dislocation on Day 24, an the tumor tissues were removed and weighe.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

Ophiopogonin D, OphiopogoninD, Ophiopogonin-D, ROS

Compound Identifiers

PubChem CID

46173859