NUP50 Antibody

• Catalog Number: orb1247185
• Category: Antibodies
• Description: NUP50 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-ELHKILLEKKDA
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NUP50
• UniProt ID: Q9UKX7
• NCBI: NP_705931.1, NP_009103.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NUP50, NPAP60, NPAP60L, MGC39961, DKFZP564A043, nu
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 60kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1ug/ml.Immunohistochemistry:In paraffin embedded Human Uterus shows textured nuclear staining of endometrial cells. Recommended concentration, 2.5-3.8ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247185 staining (0.5 ug/ml) of Jurkat lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247185 (3.8 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.