NPR3 Antibody

• Catalog Number: orb1248804
• Category: Antibodies
• Description: NPR3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-HRELREDSIRSHFS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 52 kDa
• Target: NPR3
• UniProt ID: P17342
• NCBI: NP_000899.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NPR3, natriuretic peptide receptor C/guanylate cyc
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248804 (1 ug/ml) staining of Human Placenta lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248804 (2 ug/ml) staining of paraffin embedded Human Kidney. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1248804 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb1248804 (10 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.