Natriuretic Peptide Receptor C Antibody (N-term)

• Catalog Number: orb1928596
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This Natriuretic Peptide Receptor C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 67-97 amino acids from the N-terminal region of human Natriuretic Peptide Receptor C.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: P17342
• MW: 59808 Da
• Tested applications: IF, IHC-P, WB
• Dilution range: IF: 1:200, WB: 1:1000, IHC-P: 1:10~50
• Antibody Type: Primary Antibody
• Clone Number: RB03504
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Atrial natriuretic peptide receptor 3, Atrial natr
• Note: For research use only
• NCBI: NP_001191304.1, NP_001191305.1, NP_000899.1
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-ANPC Antibody (S82) at 1:1000 dilution. Lane 1: Hela whole cell lysate. Lane 2: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 60 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Natriuretic Peptide Receptor C (NPR3/ANPC) Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of HeLa cells stained with Natriuretic Peptide Receptor C (N-term) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Natriuretic Peptide Receptor C (N-term) primary antibody (1: 200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).