NPM1 Antibody

• Catalog Number: orb1788428
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: 1-274 aa
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:1000, IHC-P: 1:25
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 32575 Da
• Target: Purified His-tagged NPM1 protein was used to produced this monoclonal antibody.
• UniProt ID: P06748
• NCBI: NP_954654.1, NP_002511.1, NP_001032827.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: NPM1;NPM; Nucleophosmin; Nucleophosmin; Nucleolar
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of paraffin-embedded H. thymus section using NPM1 Antibody. Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Western blot analysis of lysates from Hela, Jurkat, MCF-7, NIH/3T3 cell line (from left to right), using NPM1 Antibody. Diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.