Nucleophosmin/NPM1 Antibody

• Catalog Number: orb18984
• Category: Antibodies
• Description: Goat polyclonal antibody to NPM1
• Target: Nucleophosmin / NPM1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QEAIQDLWQWRKSL
• RRID: AB_10750657
• MW: 32.6; 29.5; 25.3; 18.9
• Tested applications: ELISA, IF, IHC, WB
• Dilution range: ELISA: 1:8000, WB: 0.01-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 37kDa band observed in Human HeLa lysates (calculated MW of 32.6kDa according to NP_002511.1 and 29.5kDa according to NP_954654.1). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti NPM1 antibody, anti NPM antibody, anti B23 an
• Research Area: Cancer, Epigenetics, Infectious Diseases
• Note: For research use only
• Entrez: 4869
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Mouse Spleen lysate using NPM1 antibody.

Western blot analysis of NIH3T3 (lane 1), (0.1ug/ml) Daudi (lane 2), Jurkat (lane 3) and (0.01ug/ml) K562 (lane 4) cell lysate using NPM1 antibody.

Immunofluorescence analysis of HeLa cells of NPM1 antibody.

Immunohistochemical staining of Human Testis of NPM1 antibody.