Nucleophosmin/NPM1 Antibody

SKU: orb18984

Description

Goat polyclonal antibody to NPM1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 0.01-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 37kDa band observed in Human HeLa lysates (calculated MW of 32.6kDa according to NP_002511.1 and 29.5kDa according to NP_954654.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Nucleophosmin / NPM1
• Protein Sequence: QEAIQDLWQWRKSL
• Molecular Weight: 32.6; 29.5; 25.3; 18.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NPM1 antibody, anti NPM antibody, anti B23 antibody, anti nucleophosmin (nucleolar phosphoprotein B23, numatrin) antibody, anti numatrin antibody, anti nucleolar phosphoprotein B23 antibody, anti nucleophosmin/nucleoplasmin family, member 1 antibody, anti MGC104254 antibody, anti nucleophosmin 1 antibody

Western blot analysis of Mouse Spleen lysate using NPM1 antibody.

Western blot analysis of NIH3T3 (lane 1), (0.1ug/ml) Daudi (lane 2), Jurkat (lane 3) and (0.01ug/ml) K562 (lane 4) cell lysate using NPM1 antibody.

Immunofluorescence analysis of HeLa cells of NPM1 antibody.

Immunohistochemical staining of Human Testis of NPM1 antibody.

Quick Database Links

Gene Symbol

Nucleophosmin / NPM1

NCBI Gene ID

4869