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| Catalog Number | orb1248379 |
|---|---|
| Category | Antibodies |
| Description | NPHS2 / SRN1 Antibody |
| Target | NPHS2 / SRN1 |
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Reactivity | Human, Mouse |
| Predicted Reactivity | Bovine, Porcine |
| Form/Appearance | Liquid |
| Concentration | 500 ug/mL |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Immunogen | The immunogen for this antibody is: C-SPSKPVEPLNPKK |
| UniProt ID | Q9NP85 |
| MW | Approx 45kDa |
| Tested applications | ELISA, WB |
| Application notes | Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 42-45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to Human NP_055440.1 and Mouse NP_569723.1). Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature. |
| Antibody Type | Primary Antibody |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | NPHS2, nephrosis 2, idiopathic, steroid-resistant Read more... |
| Research Area | Signal Transduction |
| Note | For research use only |
| NCBI | NP_055440.1 |
| Expiration Date | 12 months from date of receipt. |

orb1248379 (1 ug/ml) staining of Human (A) and Mouse (B) Kidney lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.
ELISA, WB | |
Bovine, Porcine | |
Human, Mouse | |
Goat | |
Polyclonal | |
Unconjugated |
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