NPHS2/SRN1 Antibody

• Catalog Number: orb131711
• Category: Antibodies
• Description: Goat polyclonal to NPHS2
• Target: NPHS2 / SRN1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SPSKPVEPLNPKK
• MW: 42.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:64000, WB: 0.5-2 μg/ml
• Application notes: Approx 45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1 μg/ml.WB: Approx 45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NPHS2; nephrosis 2, idiopathic, steroid-resistant
• Note: For research use only
• Entrez: 7827
• Expiration Date: 12 months from date of receipt.

Western blot analysis of taining of Human (A) and Mouse (B) Kidney lysates using NPHS2 antibody.

Western blot analysis of staining of Pig Kidney lysates using NPHS2 antibody.

Western blot analysis of Human (A) and Mouse (B) Kidney lysates (35ug protein in RIPA buffer) using NPHS2 antibody