NPAS4 Antibody

SKU: orb1248993

Description

NPAS4 Antibody

Research Area

Epigenetics & Chromatin, Neuroscience

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 90kDa band observed in Human Brain (Frontal Cortex) lysates and in Mouse and Rat Brain lysates (calculated MW of 87.1kDa according to NP_849195.2). Recommended concentration: 0.3-1µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: CRFNTSKSLRRQS
• Target: NPAS4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NPAS4, neuronal PAS domain protein 4, Le-PAS, NXF, PASD10, bHLHe79, HLH-PAS transcription factor NXF, PAS domain-containing protein 10, class E basic helix-loop-helix protein 79, neuronal PAS domain-containing protein 4, neuronal PAS4

orb1248993 (0.3 ug/ml) staining of Human Frontal Cortex lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1248993 (1 ug/ml) staining of Mouse (A) and Rat (B) Brain lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

NPAS4

UniProt

Q8IUM7

RefSeq (Protein):NP_849195.2