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Anti-NPAS4 Antibody

Catalog Number: orb256724

DispatchUsually dispatched within 5-10 working days
$ 140.00
Catalog Numberorb256724
CategoryAntibodies
DescriptionRabbit polyclonal antibody to NPAS4
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Porcine, Primate
ImmunogenKLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NPAS4. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetNPAS4
Entrez266743, 225872
UniProt IDQ8BGD7, Q8IUM7
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesanti BHLHE79 antibody, anti NXF antibody, anti PAS
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-NPAS4 Antibody

Western blot analysis of NPAS4 expression in U87MG (A) whole cell lysates. (Predicted band size: 87 kD; Observed band size: 87 kD)

Anti-NPAS4 Antibody

Immunohistochemical analysis of NPAS4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-NPAS4 Antibody

Immunofluorescent analysis of NPAS4 staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

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    100 μg
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    ELISA

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    Rabbit

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    50 μg, 100 μg
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    Human

    Rabbit

    Polyclonal

    FITC

    50 μg, 100 μg