NCF2 Antibody

SKU: orb1261143

Description

NCF2 Antibody

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NOXA2 / P67phox (NP_000424.2).
• Target: NCF2
• Molecular Weight: Observed: 68kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NCF-2, NOXA2, P67-PHOX, P67PHOX, neutrophil cytosol factor 2, 67 kDa neutrophil oxidase factor, NADPH oxidase activator 2, chronic granulomatous disease, autosomal 2, neutrophil NADPH oxidase factor 2, neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal 2)

Western blot analysis of extracts of various cell lines, using NOXA2 / P67phox antibody (orb1261143) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded mouse heart using NOXA2 / P67phox antibody (orb1261143) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using NOXA2 / P67phox antibody (orb1261143). Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NCF2

UniProt

P19878