NCF2/NOXA2/p67phox Antibody

• Catalog Number: orb1536007
• Category: Antibodies
• Description: NCF2/NOXA2/p67phox Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, IHC-P, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 227-526 of human NCF2 (NP_000424.2). PPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV
• Dilution range: ICC (1:20 - 1:100), IF (1:50 - 1:200), IHC (1:50 - 1:200), IHC-P (1:100), IP (1:20 - 1:100), WB (1:500 - 1:2000)
• Conjugation: Unconjugated
• Target: NCF2 / NOXA2 / p67phox
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol. PBS, pH 7.3, 0.02% Sodium Azide, 50% Glycerol
• Alternative names: NCF2, NADPH oxidase activator 2, Neutrophil cytoso
• Note: For research use only
• Application notes: Further information: The predicted MW is 47kDa/50kDa/54kDa/59kDa, while the observed MW by Western blot was 68kDa.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells using NCF2 antibody. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NCF2 antibody at 1:1000 dilution.