NOVA1 Antibody

• Catalog Number: orb1246689
• Category: Antibodies
• Description: NOVA1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-REMPQNVAKTEPVS
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NOVA1
• UniProt ID: P51513
• NCBI: NP_002506.2, NP_006480.2, NP_006482.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NOVA1, neuro-oncological ventral antigen 1, Nova-1
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246689 (0.01 ug/ml) staining of Human Breast Cancer lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

HEK293 overexpressing NOVA1 and probed with orb1246689 (mock transfection in first lane).

orb1246689 (5 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1246689 (5 ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.