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NOVA1 Antibody

SKU: orb1246689

Description

NOVA1 Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 50-55kDa band observed in Human Breast Cancer lysates (calculated MW of 51.7kDa according to NP_002506.2). In transfected HEK293 transiently expressing NOVA1 a band of approx. 52kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.01-0.03µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-REMPQNVAKTEPVS
TargetNOVA1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

NOVA1, neuro-oncological ventral antigen 1, Nova-1, paraneoplastic Ri antigen, ventral neuron-specific protein 1

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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