NOVA1 Antibody

SKU: orb19796

Description

Goat polyclonal antibody to NOVA1

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5µg/ml. Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.
• Reactivity: Human

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NOVA1
• Protein Sequence: REMPQNVAKTEPVS
• Molecular Weight: 51.7, 19.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NOVA1 antibody, anti neuro-oncological ventral antigen 1 antibody, anti Nova-1 antibody, anti paraneoplastic Ri antigen antibody, anti ventral neuron-specific protein 1 antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Primary incubation 1 hour at room temperature. Image A: Human Breast Cancer lysate at primary Ab concentration 0.03 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Flow cytometric analysis of paraformaldehyde fixed U2OS cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

5 µg/ml staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

5 µg/ml staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Quick Database Links

Gene Symbol

NOVA1

NCBI Gene ID

4857