NOTCH3 Antibody

SKU: orb629361

Description

Rabbit polyclonal antibody to NOTCH3

Images & Validation

• Tested Applications: ELISA, IHC, IP, WB
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Notch homolog 3(Drosophila)
• Target: NOTCH3
• Molecular Weight: 250-260 kDa, 100 kDa
• Purity: ≥95% as determined by SDS-PAGE
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: liquid
• Disclaimer: For research use only

COLO 320 cells were subjected to SDS PAGE followed by western blot with orb629361 (NOTCH3 antibody) at dilution of 1:500.

IP Result of anti-NOTCH3 (IP: orb629361, 4 ug; Detection: orb629361 1:500) with HeLa cells lysate 2400 ug.

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb629361 (NOTCH3 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).

Quick Database Links

Gene Symbol

NOTCH3

UniProt

Q9UM47