NOTCH3 Antibody

• Catalog Number: orb629361
• Category: Antibodies
• Description: Rabbit polyclonal antibody to NOTCH3
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Notch homolog 3(Drosophila)
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000; IP: 1:500-1:1000; IHC: 1:50-1:500
• Form/Appearance: liquid
• Purity: ≥95% as determined by SDS-PAGE
• Conjugation: Unconjugated
• MW: 250-260 kDa, 100 kDa
• Target: NOTCH3
• UniProt ID: Q9UM47
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

COLO 320 cells were subjected to SDS PAGE followed by western blot with orb629361 (NOTCH3 antibody) at dilution of 1:500.

IP Result of anti-NOTCH3 (IP: orb629361, 4 ug; Detection: orb629361 1:500) with HeLa cells lysate 2400 ug.

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using orb629361 (NOTCH3 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).