NOTCH1 Antibody

SKU: orb750484

Description

NOTCH1 antibody

Research Area

Cardiovascular Research

Images & Validation

• Tested Applications: DOT, ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:60,000, IHC: 1:200, WB: 1:500- 1:2,000
• Reactivity: Human, Mouse
• Application Notes:
  Anti-Notch 1 has been tested by ELISA, dot blot, western blot and immunohistochemistry. An 80 kDa band corresponding to Notch 1 was observed at a 1:500 dilution. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues of human Notch 1 located near the N-terminal sequence of the cleaved N intracellular domain (NICD).
• Target: NOTCH1
• Purity: This antiserum is directed against human NOTCH 1. Based on the immunogen sequence, we expect this antibody to react as well with mouse and rat NOTCH 1 (100% sequence homology). This antibody reacts with mouse Notch constructs present in lysates of HEK 293 cells. Only the cleaved intracellular (activated) form (NICD) is detected. No reactivity is detected against mouse N2, N3 or N4. The immunogen epitope is only exposed after gamma secretase cleavage and is not accessible in the uncleaved form.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Concentration: 90 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-notch1 antibody, Neurogenic locus Notch homolog protein 1, hN1, Translocation-associated Notch protein TAN-1

Quick Database Links

Gene Symbol

NOTCH1

UniProt

P46531

RefSeq:CAG33502.1