NOTCH1 Antibody

SKU: orb750482

Description

NOTCH1 antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Reactivity: Human
• Application Notes:
  Anti-Notch-1 has been tested in IHC and ELISA. This antibody is useful in western blot. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Anti-Notch antibody was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid 2480-2510 of human Notch 1. A residue of cysteine was added to the amino terminal end to facilitate coupling.
• Target: NOTCH1
• Purity: Notch1 antibody is directed against human NOTCH 1. No reaction is detected against NOTCH 2. No reactivity was observed against Mouse Notch. Other species have not been tested.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 75 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-notch1 antibody, hN1 antibody, Neurogenic locus Notch homolog protein 1 antibody, Notch 1 intracellular domain antibody, Notch homolog 1 translocation associated antibody, TAN1 antibody, Translocation associated Notch protein TAN1 antibody

Biorbyt's anti-NOTCH 1 antibody was diluted 1:500 to detect NOTCH 1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Quick Database Links

Gene Symbol

NOTCH1

UniProt

P46531

RefSeq:CAG33502.1