NKX2.1 Antibody / TTF-1

• Catalog Number: orb385837
• Category: Antibodies
• Description: Thyroid transcription factor-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. TTF-1 reactivity is also seen in thyroid malignancies.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: HBNK2-1
• Tested applications: IHC-P
• Reactivity: Human, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant protein was used as the immunogen for the TTF-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This TTF-1 antibody is available for research use only.
• UniProt ID: P43699
• Storage: Store the TTF-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titering of the TTF-1 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human thyroid tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.

IHC testing of FFPE human lung tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.

IHC testing of FFPE rat lung tissue with TTF-1 antibody (clone HBNK2-1). Required HIER: boil FFPE tissue sections in pH6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes.