NKX2.1 Antibody / TTF-1

• Catalog Number: orb606477
• Category: Antibodies
• Description: Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1, also called NKX2.1. TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rNX2.1/690
• Tested applications: IHC-P
• Reactivity: Human, Mouse, Rat
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant protein was used as the immunogen for the recombinant TTF-1 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant TTF-1 antibody is available for research use only.
• UniProt ID: P43699
• Storage: Store the recombinant TTF-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant TTF-1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC: Formalin-fixed, paraffin-embedded human thyroid stained with recombinant TTF-1 antibody (clone rNX2.1/690). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

IHC: Formalin-fixed, paraffin-embedded human lung adenocarcinoma stained with recombinant TTF-1 antibody (clone rNX2.1/690). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant TTF-1 antibody (clone rNX2.1/690) as confirmation of integrity and purity.