NKX2-1 Antibody

• Catalog Number: orb1239361
• Category: Antibodies
• Description: NKX2-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: NKX2-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human NKX2-1.The immunogen is located within the first 50 amino acids of NKX2-1.
• Concentration: 1 mg/mL
• Dilution range: NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 46 kDa Observed: 41, 45 kDa
• Target: NKX2-1
• UniProt ID: P43699
• NCBI: P43699
• Storage: NKX2-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: NKX2-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: NKX2-1 Antibody: BCH, BHC, NK-2, TEBP, TTF1, NKX2A
• Note: For research use only
• Application notes: NKX2-1 antibody can be used for detection of NKX2-1 by Western blot at 0.25 – 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NKX2-1 expression in human lung tissue lysate with NKX2-1 antibody at (A) 0.25 and (B) 0.5 µg/ml.

Immunohistochemistry of NKX2-1 in human lung tissue with NKX2-1 antibody at 2.5 µg/mL.

Immunofluorescence of NKX2-1 in human lung tissue with NKX2-1 antibody at 20 µg/mL.