TTF1/NKX2-1 Rabbit Polyclonal Antibody

• Catalog Number: orb11533
• Category: Antibodies
• Description: TTF1/NKX2-1 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus, Sheep
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human TTF-1 (201-300/372aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:200-1000, IHC-P=1:50-200, Flow-Cyt=1μg/Test, IHC-F=1:50-200, IF=1:50-200, ICC/IF=1:50-1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 38 kDa
• Target: NKX2-1
• UniProt ID: P43699
• RRID: AB_10746411
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NKX21_HUMAN; Homeobox protein Nkx-2.1; Homeobox pr
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Lane 1: Mouse Thyroid gland Lysates, Lane 2: Rat Thyroid gland Lysates, Primary: Anti-TTF1 (orb11533) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 38kDa, Observed band size: 38kDa.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-TTF1/NKX2.1 Polyclonal Antibody, Unconjugated (orb11533) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.