NKCC2 Antibody

• Catalog Number: orb67565
• Category: Antibodies
• Description: Rabbit Anti-Rat NKCC2 Polyclonal
• Target: NKCC2
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Purification: Protein A Purified
• Immunogen: Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody desgination L224).
• UniProt ID: Q5PQV1
• MW: 160kDa
• Tested applications: IHC, WB
• Dilution range: WB (1:1000); IHC-P (1:25)
• Application notes: 1 µg/ml of SPC-401 was sufficient for detection of NKCC2 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: SLC12A1 Antibody, sodium potassium chloride cotran
• Note: For research use only
• Entrez: 25065
• NCBI: NP_001257546.1

Immunohistochemistry analysis using Rabbit Anti-NKCC2 Polyclonal Antibody. Tissue: Kidney. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NKCC2 Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody at 1:1000.