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NKCC2 Antibody
SKU: orb67565
Description
Research Area
Cancer Biology, Neuroscience, Pharmacology & Drug Discovery
Images & Validation
−Item 1 of 2
| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | WB (1:1000); IHC-P (1:25) |
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Produced against a synthetic peptide mapping to a segment (amino acids 33-55) of the N-terminal tail of rat NKCC2 (antibody designation L224). |
| Target | NKCC2 |
| Molecular Weight | 160kDa |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS, 50% glycerol, 0.09% sodium azide Storage buffer changes when conjugated |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SLC12A1, sodium potassium chloride cotransporter 2, sodium-potassium-chloride cotransporter isoform 2, BSC1, MBSC1, Na-K-2Cl cotransporter, NKCC2A variant A, RBSC
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Immunohistochemistry analysis using Rabbit Anti-NKCC2 Polyclonal Antibody. Tissue: Kidney. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NKCC2 Antibody (D) Composite
Western blot analysis of Rat kidney tissue lysates showing detection of NKCC2 protein using Rabbit Anti-NKCC2 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NKCC2 Polyclonal Antibody at 1:1000.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Gene Details
− No NCBI Gene data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001257546.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
NKCC2 Antibody (orb67565)
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