NHEJ1 Antibody

SKU: orb1257619

Description

NHEJ1 Antibody

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-299 of human NHEJ1 (NP_079058.1).
• Target: NHEJ1
• Molecular Weight: Observed: 38kDa
• Purification: Affinity purification

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Concentration: batch dependent
• Disclaimer: For research use only

Alternative Names

NHEJ1, nonhomologous end-joining factor 1, FLJ12610, XLF, Cernunnos, XRCC4-like factor

Western blot analysis of extracts of various cell lines, using NHEJ1 antibody (orb1257619) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded human breast cancer using NHEJ1 antibody (orb1257619) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterine cancer using NHEJ1 antibody (orb1257619) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of C6 cells using NHEJ1 antibody (orb1257619) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2OS cells using NHEJ1 antibody (orb1257619) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

NHEJ1

UniProt

Q9H9Q4