XRCC4-like factor/NHEJ1 Antibody

SKU: orb19337

Description

Goat polyclonal antibody to NHEJ1

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5-3.8µg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 38kDa band observed in lysates of Human Skin, Testis and Thyroid gland (calculated size of 33.3kDa according to NP_079058.1). The observed molecular weight corresponds to findings with antibodies from other sources. The 38kDa band was successfully blocked by incubation with the immunizing peptide. Recommended concentration 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: XRCC4-like factor / NHEJ1
• Molecular Weight: 33.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti NHEJ1 antibody, anti XRCC4-like factor antibody, anti nonhomologous end-joining factor 1 antibody, anti FLJ12610 antibody, anti XLF antibody, anti Cernunnos antibody

3.8 µg/ml staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH6, AP-staining.

Primary incubation 1 hour at room temperature.Image A: HeLa nuclear cell lysate at primary Ab concentration 0.01 µg/ml, Images B, C, D: A431, Jurkat, K562 nuclear cell lysate at primary Ab concentration 0.03 µg/ml, Image E: HepG2 nuclear cell lysate at primary Ab concentration 0.1 µg/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Primary incubation 1 hour at room temperature. Image A: Human Skeletal muscle lysate at primary Ab concentration 0.03 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

XRCC4-like factor / NHEJ1

NCBI Gene ID

79840