NHE3 Antibody

• Catalog Number: orb67564
• Category: Antibodies
• Description: Rabbit Anti-Rat NHE3 Polyclonal
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Mouse, Rat
• Immunogen: Synthetic peptide mapping to AA 809 to 831 of rat sequence
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC-P (1:25)
• Conjugation: Unconjugated
• MW: 84kDa
• Target: NHE3
• Entrez: 24784
• UniProt ID: P26433
• NCBI: NP_036786
• Storage: -20°C
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Alternative names: Sodium / Hydrogen Exchanger 3 antibody, SLC9A3 Ant
• Note: For research use only
• Application notes: 1 µg/ml of SPC-400 was sufficient for detection of HNE3 in 10 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-NHE3 Polyclonal Antibody. Tissue: Colon. Species: Rat. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit IgG:Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) NHE3 Antibody (D) Composite

Western blot analysis of Rat kidney tissue lysates showing detection of NHE3 protein using Rabbit Anti-NHE3 Polyclonal Antibody. Primary Antibody: Rabbit Anti-NHE3 Polyclonal Antibody at 1:1000.