Nhe-1 Antibody

• Catalog Number: orb1239376
• Category: Antibodies
• Description: Nhe-1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Nhe-1 antibody was raised against a 20 amino acid synthetic peptide near the carboxy terminus of the human Nhe-1.The immunogen is located within the last 50 amino acids of Nhe-1.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 52, 90 kDa Observed: 50, 90 kDa
• Target: SLC9A1
• UniProt ID: P19634
• NCBI: P19634
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Nhe-1 Antibody: APNH, NHE1, NHE-1, APNH1, APNH
• Note: For research use only
• Application notes: Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Nhe-1 in rat kidney tissue lysate with in with Nhe-1 antibody at (A) 1 and (B) 2 µg/mL.

Immunohistochemical staining of human brain tissue using Nhe-1 antibody at 2.5 µg/mL.

Immunofluorescence of Nhe-1 in Human Brain tissue with Nhe-1 antibody at 20 µg/mL.