SLC9A1 Antibody

• Catalog Number: orb1239375
• Category: Antibodies
• Description: SLC9A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC-P, WB
• Predicted Reactivity: Bovine, Porcine, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti-Nhe-1 antibody was raised against a peptide corresponding to 17 amino acid synthetic peptide near the center of human Nhe-1. The immunogen is located within amino acids 49-540 of Nhe-1.
• Antibody Type: Primary Antibody
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 91 kD Observed: 91 kD
• Target: SLC9A1
• UniProt ID: P19634
• NCBI: P19634
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Nhe-1 Antibody: APNH, NHE1, NHE-1, APNH1, APNH
• Note: For research use only
• Application notes: WB: 1-4 μg/mL; IHC-P: 1-2 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

WB Validation in K562 Cells. Loading: 10 μg of lysate. Antibodies: Nhe-1, orb1239375, 4 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Tissues. Loading: 15 μg of lysate. Antibodies: Nhe-1, orb1239375, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Brain. Loading: 15 μg of lysate. Antibodies: Nhe-1, orb1239375, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of Nhe-1 in Human Small Intestine. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nhe-1 antibody (orb1239375) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Nhe-1 in Mouse Colon. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Nhe-1 antibody (orb1239375) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Nhe-1 in Rat Small Intestine. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Nhe-1 antibody (orb1239375) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.