SLC9A1 Antibody

SKU: orb1239375

Description

SLC9A1 Antibody

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine, Rabbit
• Application Notes:
  Nhe-1 antibody can be used for detection of Nhe-1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Nhe-1 antibody was raised against a 17 amino acid synthetic peptide near the center of the human Nhe-1.The immunogen is located within amino acids 490 - 540 of Nhe-1.
• Target: SLC9A1
• Molecular Weight: Predicted: 91 kD Observed: 91 kD
• Purification: Nhe-1 Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Nhe-1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Disclaimer: For research use only

Alternative Names

Nhe-1 Antibody: APNH, NHE1, NHE-1, APNH1, APNH

WB Validation in K562 Cells. Loading: 10 μg of lysate. Antibodies: Nhe-1, orb1239375, 4 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Mouse Tissues. Loading: 15 μg of lysate. Antibodies: Nhe-1, orb1239375, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

WB Validation in Rat Brain. Loading: 15 μg of lysate. Antibodies: Nhe-1, orb1239375, 1 μg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of Nhe-1 in Human Small Intestine. Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-Nhe-1 antibody (orb1239375) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Nhe-1 in Mouse Colon. Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Nhe-1 antibody (orb1239375) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunohistochemistry Validation of Nhe-1 in Rat Small Intestine. Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Nhe-1 antibody (orb1239375) at 1 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Quick Database Links

Gene Symbol

SLC9A1

UniProt

P19634

RefSeq:P19634