NFM (Neurofilament Medium Chain) Antibody

SKU: orb3167278

Description

NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980). Biochem. J. 191(2): 543-546], followed by Prepcell purification (Bio-Rad). Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.

Images & Validation

• Tested Applications: ICC, IHC, WB
• Dilution Range: WB: 1:1000-1:2000; IHC: 1:1000-1:2000; ICC: 1:1000-1:2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Gallus
• Clonality: Polyclonal
• Isotype: IgY
• Immunogen: Recombinant construct containing the C-terminus of the human sequence expressed in Escherichia coli
• Target: Neurofilament Medium Chain (NFM)
• Molecular Weight: 150 kDa
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS" pH 7.2) with rAlbumin (rAlbumin, 0.5%) added to prevent absorption to the plastic and sodium azide (0.02%, w/v) added as a preservative
• Concentration: 2 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein)

Quick Database Links

Gene Symbol

Neurofilament Medium Chain (NFM)

UniProt

P07197