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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB: 1:500-1:2000; IP: 1:500-1:2000; IHC: 1:50-1:500; IF: 1:50-1:500 |
| Reactivity | Human, Mouse, Porcine, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha |
| Target | NFKBIA |
| Molecular Weight | 39 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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HL-60 cells were subjected to SDS PAGE followed by western blot with orb627966 (NFKBIA antibody) at dilution of 1:500.

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using orb627966 (NFKBIA Antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).

IP Result of anti-IkB alpha (IP: orb627966, 4 ug; Detection: orb627966 1:1000) with HeLa cells lysate 4000 ug.

Immunohistochemistry of paraffin-embedded human kidney tissue slide using orb627966 (NFKBIA Antibody) at dilution of 1:200.
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NFKBIA Rabbit Polyclonal Antibody (orb627966)
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