Phospho-IKB alpha (Ser32 + Ser36) Rabbit Polyclonal Antibody

• Catalog Number: orb13487
• Category: Antibodies
• Description: Phospho-IKB alpha (Ser32 + Ser36) Rabbit Polyclonal Antibody
• Target: NFKBIA
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine, Rabbit, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human IKB alpha around the phosphorylation site of Ser32/36 HD(p-S)GLD(p-S)M
• UniProt ID: P25963
• RRID: AB_10749038
• MW: 35 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IKB alpha(phospho S32+S36); p-IKB alpha(phospho S3
• Note: For research use only

References

Wu, Zhenhui et al. Total C-21 steroidal glycosides, isolated from the root tuber of Cynanchum auriculatum Royle ex Wight, attenuate hydrogen peroxide-induced oxidative injury and inflammation in L02 cells Int J Mol Med, 42, 3157-3170 (2018)

PubMed: 30272289

Applications

IHC

Reactivity

Human

Deng, Dan et al. Silencing MEG3 protects PC12 cells from hypoxic injury by targeting miR-21 Artif Cells Nanomed Biotechnol, 48, 610-619 (2020)

PubMed: 32050796

Applications

WB

Reactivity

Rat

HepG2 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-IKB alpha (Ser32 + Ser36)) polyclonal Antibody, Unconjugated (orb13487) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Paraformaldehyde-fixed, paraffin embedded (Rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-IKB alpha (Ser32 + Ser36)) Polyclonal Antibody, Unconjugated (orb13487) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug, Lane 2: Hela (Human) Cell Lysate at 30 ug, Lane 3: Siha (Human) Cell Lysate at 30 ug, Lane 4: U251 (Human) Cell Lysate at 30 ug, Primary: Anti-phospho-IKB alpha (Ser32 + Ser36) (orb13487) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 39 kD, Observed band size: 37 kD.