NFKB1 Antibody

SKU: orb750585

Description

NFKB1 antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:5,000 - 1:25,000, WB: 1:500 - 1:2,000
• Reactivity: Human
• Application Notes:
  This product was tested by immunoblot and ELISA. Anti-Human NFkB p50 (NFKB1) is suitable for the detection by immunoblot of Human NFkB p50 (NFKB1) and its precursor protein p105, and was found to be reactive against Human NFkB p50 (NFKB1) at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) . No reaction was observed against the analogous mouse protein. This product was also tested in a gel supershift assay and found to be reactive against p50:p50 homodimers and p:50:p65 heterodimers using 0.5 to 1.0 µl per assay.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Antiserum
• Immunogen: Human NFkB p50 (NFKB1) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
• Target: NFKB1
• Purity: This product was prepared from monospecific antiserum by delipidation and defibrination. Anti-Human NFkB p50 (NFKB1) may react non-specifically with other proteins. Control peptide (code #100-4164p) will compete only with the specific reaction of antiserum with Human NFkB p50 (NFKB1).
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 80 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit Anti-NFKB p50 antibody, rabbit anti-NFKB1 antibody, NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB

Quick Database Links

Gene Symbol

NFKB1

UniProt

P19838

RefSeq:CAB94757.1