NFKB1 Rabbit Polyclonal Antibody

• Catalog Number: orb11124
• Category: Antibodies
• Description: NFKB1 Rabbit Polyclonal Antibody
• Target: NFKB1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Gallus, Porcine
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Nuclear factor NF-kappa-B p50 subunit (51-100/968aa)
• UniProt ID: P19838
• RRID: AB_10752497
• MW: 48/105 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: NFKB1_HUMAN; Nuclear factor NF-kappa-B p105 subuni
• Research Area: DNA and RNA Research, Inflammation, Macrophage, NF
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Oskoueian, Ehsan et al. Palm kernel cake extract exerts hepatoprotective activity in heat-induced oxidative stress in chicken hepatocytes BMC Complement Altern Med, 14, 368 (2014)

PubMed: 25273634

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NFkB p50) Polyclonal Antibody, Unconjugated (orb11124) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat bladder), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (orb11124) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (orb11124) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat uterus), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (NFkB p105) Polyclonal Antibody, Unconjugated (orb11124) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Brain (Mouse) Lysate at 30 ug, Primary: Anti-NFkB p105/p50 (orb11124) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 48/105 kD, Observed band size: 50 kD.

Sample: Lane 1: Human Raji cell lysates, Lane 2: Human MCF-7 cell lysates, Lane 3: Human Jurkat cell lysates, Primary: Anti-NFKB1 (orb11124) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 48/105 kDa, Observed band size: 135 kDa.

Sample: Lung (Mouse) lysate at 30 ug, Primary: Anti-NFkB p105/p50 (orb11124) at 1:300 dilution, Secondary: HRP conjugated Goat-Anti-rabbit IgG (orb572747) at 1:5000 dilution, Predicted band size: 48/105 kD, Observed band size: 110 kD.