NFIL3 Antibody

• Catalog Number: orb1247202
• Category: Antibodies
• Description: NFIL3 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RLIATQPISASDSG
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: NFIL3
• UniProt ID: Q16649
• NCBI: NP_005375.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: NFIL3, nuclear factor, interleukin 3 regulated, E4
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx. 60kDa band observed in nuclear lysates of cell line Jurkat and HeLa (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to different antibodies from other commercial sources. An additional band of 30kDa was observed in lysates of cell line HeLa, which was completely blocked with the immunizing peptide. Recommended concentration: 0.5-2ug/ml. Primary incubation 1 hour at room temperature.Flow Cytometry: Flow cytometric analysis of Human peripheral blood mononuclear cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247202 staining (1 ug/ml) of Jurkat nuclear cell lysate (RIPA buffer, 20ug total protein per lane). Detected using chemiluminescence.

orb1247202 Flow cytometric analysis of paraformaldehyde fixed Human peripheral blood mononuclear cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).