NFIL3 Antibody

SKU: orb18303

Description

Goat polyclonal antibody to E4BP4

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:2000, WB: 0.5-2 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 55-60kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2609-14.; PMID: 9122243). Recommended concentration: 0.5-1.5 μg/ml.

  Read more...

Key Properties

• Clonality: Polyclonal
• Target: NFIL3
• Protein Sequence: RLIATQPISASDSG
• Molecular Weight: 51.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-E4BP 4 antibody, anti-E4BP4 antibody, anti-E4BP4 protein antibody, anti-NFIL 3 antibody, anti-NFIL 3A antibody, anti-NFIL3 antibody, anti-NFIL3_HUMAN antibody, anti-NFIL3A antibody, anti-Nuclear factor interleukin 3 regulated antibody, anti-Nuclear factor interleukin-3-regulated protein antibody

Western blot analysis of HepG2 cell lysate using NFIL3 antibody

Quick Database Links

Gene Symbol

NFIL3

NCBI Gene ID

4783