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NFIL3 Antibody

SKU: orb18303

Description

Goat polyclonal antibody to E4BP4

Images & Validation

Tested ApplicationsELISA, FC, WB
Dilution rangeELISA: 1:2000, WB: 0.5-2 μg/ml, IHC-P: 10ug/ml
ReactivityHuman
Predicted ReactivityMouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx. 55-60kDa band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kDa according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2609-14.; PMID: 9122243). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetNFIL3
Molecular Weight51.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

Anti-E4BP 4 antibody, anti-E4BP4 antibody, anti-E4BP4 protein antibody, anti-NFIL 3 antibody, anti-NFIL 3A antibody, anti-NFIL3 antibody, anti-NFIL3_HUMAN antibody, anti-NFIL3A antibody, anti-Nuclear factor interleukin 3 regulated antibody, anti-Nuclear factor interleukin-3-regulated protein antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NFIL3 Antibody

Western blot analysis of HepG2 cell lysate using NFIL3 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

NFIL3 Antibody (orb18303)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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